Coding

Part:BBa_K3809006:Design

Designed by: Francisco Javier Castañeda Villagran   Group: iGEM21_TecCEM   (2021-10-07)


PuDHT


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 238
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 981
    Illegal NgoMIV site found at 1194
    Illegal NgoMIV site found at 1602
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 265


Design Notes

The sequence was codon optimized for E. coli.


Source

The sequence was obtained from Paralcaligenes ureilyticus strain DSM 24591 Ga0244690_119, NCBI Reference Sequence, NZ_SMAJ01000019.1

References